Coding

Part:BBa_K909008:Design

Designed by: Gintautas Vainorius   Group: iGEM12_ETH_Zurich   (2012-09-23)


tetR-DBD-UVR8 fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 418
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 382
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 433


Design Notes

Contains two illegal PstI sites in UVR8 coding region and BamHI site was used for protein fusion


Source

tetR-DBD was cloned from E.coli tetracyclin repressor tetR from transposon Tn10 cDNA of UVR8 was provided by Roman Ulm, University of Geneva.

References